![]() ![]() ![]() However, most of the faces in that game are found in the N64 version, and the wives are also the same. In Harvest Moon: A Wonderful Life, You must get Married, raise a Family in a whole new Setting. The Game is actually 30 Years long, but you only get to play 10 of them. Each Year is 40 Days long, 10 Days in each Season. This Game is English (USA) Version and is the highest. In this Version of Harvest Moon, you play 10 Years. The town has essentially been deserted, but you're never one to back down from a challenge! It will be up to you to help rebuild the town and save the lighthouse. Release Date: Summary Critic Reviews User Reviews Details & Credits Trailers & Videos Metascore 79 Generally favorable reviews based on 28 Critic Reviews What's this User Score 8. Harvest Moon: Back to Nature was the first Harvest Moon to be released on a non-Nintendo console, and it is also considered to be a great one. Harvest Moon: A Wonderful Life ROM for GameCube download requires an emulator to play the game offline. Looking for a fresh start and some new surroundings, you set off on a voyage to begin your new life! Unfortunately, the weather has different plans, as your ship is hit by a monsoon, and goes down! You drift into a small harbor town, now in shambles from the storm, where a young doctor named Jeanne saves your life. ![]() Picture might be for reference and not the actual item: item. In celebration of Harvest Moon's 20th Anniversary comes an all new Harvest Moon title for Steam! Harvest Moon: Light of Hope Special Edition! The game encompasses twenty years of the spirit that have made the franchise what it is today! Harvest Moon A Wonderful Life (Nintendo Gamecube). ![]()
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![]() ![]() White space, or the places in your resume without words or formatting, adds breathing room to your resume. But nothing works quite as well as more white space. Headings and call-out boxes can help with this problem. Often times, candidates are so focused on adding every little detail to their resume that it becomes too crammed to read. Strategically-Placed White Space and Tight Copy Then these call-out boxes can be used to show off your best features, as they’re likely one of the first things hiring managers will notice during their initial scan. Your resume headings and sections will create an organized outline of your qualifications. So think of your resume as a cheat sheet for hiring managers and recruiters rather than a dossier. This is like telling hiring teams, “Hey! Come check this out!” The highlights and expertise section in this resume template sits in a blue box to draw the reader’s eyes and attention. The example below takes this concept a step further: The resume template above does a fantastic job of breaking up a resume into bite-sized chunks of information. ![]() This makes your resume easier to scan and read, not to mention far more enjoyable for busy hiring managers. That’s why most modern resume templates are divided into sections with clear, distinct headers. And with hundreds of other resumes vying for their attention, they’ll probably just pass on doing so. They tell hiring teams they’ll need to spend time and effort to mine for the intel they’re looking for. Resumes that look like one giant block of text are intimidating. Clear Sections To Highlight Your Best Features Rather than reading like a bland job description, each point should back up your skill set while proving your track record of success.Īnyone reading your resume formatted with a modern template like this will know precisely who you are and the value you’ll deliver, which is the ultimate goal. So the bottom half of this resume template helps organize your career achievements in an engaging, eye-appealing way. Readers will then feel compelled to learn where you honed those skills. A quick scan of the top half provides them with a snapshot of your areas of expertise and core competencies, two very attractive “hooks” to hiring teams. Unlike a traditional resume template, the one above builds a personal connection with readers by introducing and showcasing your value first. Want to use this resume template? Click this link purchase it and start customizing this template (or any others in this article) now. That’s why it’s so important to freshen up the look of your resume with an updated, modern resume template like this one: So your resume could be passed over in that brief 7.4-second scan before it’s even read. These boring resumes don’t excite or connect with readers. See, hiring managers tune out when one of those stale resume templates lands on their desks for the umpteenth time. An Updated, Modern DesignĪ traditional resume format (think: chronological order, bullet points, etc.) won’t give you a leg-up on your competition in 2023. ![]() So every resume in 2023 should incorporate these five elements to achieve that goal: 1. It subtly draws them down the page to learn why you’re the candidate they’re looking for. What Your Resume Should Look Like In 2023: 5 Must-Haves + ExamplesĪn attractive, high-performing resume immediately piques a reader’s interest. ![]() ![]() ![]() Hand and forearm straps can sometimes be used for other purposes. They can move heavy, bulky items, and that’s about it. Versatilityįull back braces and shoulder moving straps have limited uses. If the load feels too heavy, even with moving straps, you may need extra hands to move the item. It’s important to maintain good posture while moving heavy items, lifting with the legs. The strap’s maximum weight capacity may be 800 to 1,000 pounds, but the users’ capacity may only be 500 pounds. Keep in mind that though the maximum weight load may be very high, whether or not the item can be moved depends on how much weight the strap’s users can lift. A few full back braces and shoulder moving straps can carry up to 1,000 pounds. Each person wears a harness or forearm strap on either side of the object being moved. Moving straps with weight capacities over 600 pounds are typically two-person moving strap systems with shoulder or back braces. Some forearm straps allow movers to carry up to 800 pounds. Hand straps and forearm straps can usually bear weights between 400 to 660 pounds. Moving straps’ maximum weight capacities vary according to the straps’ style and design. Moving straps with adjustable harnesses or chest straps are particularly useful when the two people using the straps are of different heights or sizes because they help distribute the load’s weight evenly. ![]() The strap’s adjustability also contributes to the users’ comfort. Padded straps and harnesses prevent the straps from cutting into your skin. A wider strap-one that’s 3 to 5 inches wide-distributes weight over a larger surface area, making the strap more comfortable to use. However, the comfort of a strap often comes down to how wide and well padded it is. To accommodate users of different heights and sizes, most moving straps are adjustable to some degree. When using moving straps with metal buckles, it’s not a bad idea to wrap furniture and other goods with a moving blanket to prevent them from being scratched by the metal. Some moving straps have buckles that attach to a harness, and others have heavy-duty metal buckles with locking mechanisms to prevent slipping. The extra stitching prevents the strap from stretching or breaking when the load gets heavy. It’s not just the quality of the strap material that matters, as finishing touches like double stitching and reinforced seams make a difference too. Moving straps are typically made of heavy-duty nylon, though the ones with full back braces may have cotton duck in the harness. Their weight capacity is similar to that of shoulder moving straps. These harnesses are usually well padded, which makes them more comfortable to wear than shoulder braces. Full back brace: A full back brace moving strap has a harness that fits behind the back, dispersing weight across the upper back and shoulders.Some designs include padded straps to keep them from digging into the shoulders. ![]() Shoulder moving straps distribute weight evenly on the shoulders, leaving at least one arm free to balance the object or open doors. Some also connect behind the back or have a chest strap to make carrying more stable. Shoulder: Shoulder moving straps, or braces, have a strap or harness that fits over each shoulder.Forearm braces require two people to use-one on each end of the straps-and allow you to use various strap configurations to suspend heavy items between you and a partner. These straps help movers carry heavy weight closer to the body, thus reducing strain on the back. Forearm: A forearm moving strap (or brace) has a loop, or loops, on both ends of the strap that fit over the forearms.These straps are typically the least expensive, have the lowest weight capacity, and are meant to be used by just one person. Some can be adjusted to fit under items of different lengths and sizes. Hand Straps: The simplest kind of moving strap is basically a harness with a handle on each end.Before investing in moving straps, learn more about how much weight they can hold and the best types of straps for the items you need to move. Moving straps aren’t complicated, but choosing the right type can really affect how smoothly and efficiently your move goes. Photo: What to Consider When Choosing the Best Moving Straps ![]() ![]() Sending this information through emails, text messages, or even communication platforms like Slack is inherently insecure since these methods also lack encryption. Unlike the best password managers, Chrome doesn’t have a secure sharing portal to safely transfer passwords and other private information. On the other hand, if encrypted passwords are intercepted, lost, or stolen, they’re essentially useless to hackers. Anyone using your device can easily access the credentials saved in Chrome or any other browser password manager with minimal effort. This is helpful for cybercriminals and potentially disastrous for you. Is Google’s built-in password manager safe? When the Chrome browser password manager is operating, your username and password will appear in an unencrypted list on your desktop, meaning the data isn’t scrambled to protect it from unauthorized access. Using multiple browsers or devices limits the usefulness of the password vault and makes your logins less portable. For example, the information you’ve saved to Chrome won’t be synced to Safari on your iPhone, and vice versa. That means you don’t have access to your saved passwords when using a different browser or app on your desktop or device. The Chrome browser password manager only works when you’re using Chrome. ![]() Once you erase the file, be sure to empty the trash on your computer as well. Important: After you’ve imported your passwords into a secure standalone password manager like Dashlane and confirmed all of your passwords have migrated over properly, delete the CSV file from your computer to ensure it never falls into the wrong hands. This last step is the most important since Dashlane’s Autofill feature won’t work otherwise. Finally, now that your logins are saved to Dashlane, you can delete the CSV file from your device, erase saved passwords from your browser, and turn off its password manager feature.If everything looks correct, select “Import items.” Dashlane will confirm how many items you’ve imported.If you have a Starter, Team, or Business plan, you’ll also be asked whether to import your data to your Personal or Business Space. You can choose whether to put an item into “Logins” or “Secure Notes” and decide which ones not to import at all. Select “Next” to preview your file in Dashlane. ![]()
![]() ![]() Stranger Things is set in the fictional rural town of Hawkins, Indiana, in the 1980s. An animated spin-off series, developed by Eric Robles and produced by Flying Bark Productions, is in development. It has received numerous nominations and awards. It has been critically acclaimed for its characterization, atmosphere, acting, soundtrack, directing, writing, and homages to 1980s films. One of Netflix's flagship series, Stranger Things has attracted record viewership on the streaming platform. They also took inspiration from experiments conducted during the Cold War and conspiracy theories involving secret government programs. Several thematic and directorial elements were inspired by the works of Steven Spielberg, John Carpenter, David Lynch, Stephen King, Wes Craven and H. The Duffer Brothers developed Stranger Things as a mix of investigative drama and supernatural elements portrayed with horror and childlike sensibilities, while infusing references to the pop culture of the 1980s. ![]() The ensemble cast includes Winona Ryder, David Harbour, Finn Wolfhard, Millie Bobby Brown, Gaten Matarazzo, Caleb McLaughlin, Natalia Dyer, Charlie Heaton, Cara Buono, Matthew Modine, Noah Schnapp, Sadie Sink, Joe Keery, Dacre Montgomery, Sean Astin, Paul Reiser, Maya Hawke, Priah Ferguson and Brett Gelman. Set in the 1980s, the series centers around the residents of the fictional small town of Hawkins, Indiana, as they are plagued by a hostile alternate dimension known as the Upside Down, after a nearby human experimentation facility opens a gateway between it and the normal world. In February 2022, Stranger Things was renewed for a fifth and final season. The second and third season followed in October 2017 and July 2019 respectively, and the fourth season aired in two parts in May and July 2022. ![]() Produced by Monkey Massacre Productions and Levy's 21 Laps Entertainment, the first season was released on Netflix on July 15, 2016. Stranger Things is an American science fiction horror drama television series created by the Duffer Brothers, who also serve as showrunners and are executive producers along with Shawn Levy and Dan Cohen. ![]() ![]() ![]() Lysates containing nuclear extracts should also be passed through a needle, sonicated, or treated with endonuclease to break down DNA and carbohydrates and reduce sample viscosity. If a detergent-free lysis buffer is used, the sample should be homogenized using a Dounce homogenizer, sonicated, or passed through a 28-gauge needle. High salt concentrations can also be used to promote protein solubility, although excess salt can produce wavy bands during electrophoresis. Many proteins are soluble at a higher pH. If native lysis conditions are required, proteins may be solubilized by varying the pH and ionic strength of the lysis buffer. RIPA or perform mitochondrial fractionation. Table 3: Select an appropriate lysis buffer based on the target protein’s localization. In some cases, chaotropic agents that disrupt hydrogen bonding, such as 8M urea, can be used to isolate difficult to solubilize proteins, however, these samples should not be heated as urea can be hydrolyzed to cyanate, which can chemically modify the protein sample. RIPA (radioimmunoprecipitation assay) buffer, which contains SDS, is well suited for preparing whole cell extracts, membrane-bound extracts, and nuclear extracts, but disrupts protein-protein interactions. Zwitterionic detergents, such as CHAPS, are better able to solubilize membrane proteins. NP-40 buffer or Triton X-100-containing buffer can be used to prepare whole cell extracts, cytoplasmic extracts, and membrane-bound extracts, however, hydrophobic proteins may remain insoluble under these conditions. The epitope information and / or suggested immunoblotting conditions should be indicated on the antibody’s data sheet.ĭistinct subcellular fractions of a biological sample can be isolated by varying the strength of the detergent in the lysis buffer (Table 3). In general, denaturing conditions are used for cell lysis, however, if the antibody only recognizes the target protein in its native state, do not use SDS in the electrophoresis procedure and do not heat the sample. ![]() The formulation of the lysis buffer will depend on the subcellular location of the protein of interest, as well as on whether the antibody’s epitope is still present on the protein once it has been denatured. A specific protein of interest is then detected on the membrane using antibodies that recognize an epitope in the target protein, horseradish peroxidase (HRP)-conjugated secondary antibodies, and a chemiluminescent-based detection substrate. The proteins are then transferred to a solid-phase membrane. The sample is then subject to gel electrophoresis allowing the proteins in the mixture to be separated according to their size. In this example, a lysate sample is prepared from cultured cells. Western blotting relies on the electrophoretic separation of proteins from a complex mixture based on their mass, the transfer of these proteins to a solid matrix, and the detection of specific proteins of interest on the matrix using antibodies.įigure 1: The flow-through of a typical western blot experiment. The western blot, also commonly known as immunoblot, has since become an essential and ubiquitous technique in biology and medical labs around the world. The research groups of George Stark at Stanford University and Harry Towbin at Friedrich Miescher Institut in Switzerland published similar immunoblotting techniques at roughly the same time. Despite this, the paper was widely circulated and eventually published in 1981. Interestingly, the manuscript detailing this technique was initially rejected, with reviewers criticizing the method’s name as “flippant and frivolous whimsy”. He subsequently termed this method the “western blot”, in a nod to its predecessors. Neal Burnette, a postdoc at the Fred Hutchinson Cancer Research Center, developed a method for visualizing proteins separated by SDS-PAGE using monoclonal antibodies. This method was quickly followed two years later by the invention of the “northern blot”, which could detect specific RNA molecules using radio-labeled DNA probes. In 1975, Edwin Southern invented the eponymously-named “southern blot”, a technique in which DNA fragments are separated through electrophoresis based on their size and then transferred to a nitrocellulose membrane for detection. In fact, essential western blot methodology, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and monoclonal antibodies with antigen specificity, were only established in 19, respectively. It may be surprising to learn that the history of the western blot only stretches back to the late 1970s. ![]() ![]() ![]() A ligament called the ligament of Struthers is present in 1 percent to 3 percent of the population. This location is only a possible source of nerve entrapment in a small percentage of the population. Moving distally after leaving the axillary region, the next location where median nerve entrapment is likely, is just proximal to the elbow. Other thoracic outlet syndrome variations that may compress the median nerve include the region between the anterior and middle scalene muscles, between the clavicle and first rib, and underneath the pectoralis minor muscle. ![]() This is called true neurological thoracic outlet syndrome. Fibers of the median nerve can get compressed against a pathological bony extension of the C7 transverse process, called a cervical rib. Thoracic outlet syndrome is not consistently defined in the medical literature, so there is a great deal of confusion about it. Since the nerve roots also contain fibers for other peripheral nerves, symptoms of compression at the nerve root level may extend outside the commonly mapped area for median nerve sensory involvement illustrated in Figure 1.Īfter leaving the cervical region, the next several locations of potential entrapment are all part of what is commonly called thoracic outlet syndrome. Intervertebral discs, bone spurs, small tumors, or other obstructions may press on these nerve roots and produce symptoms that affect the median nerve. The median nerve is derived from the C5-T1 nerve roots. The first location where median nerve compression may occur is at the cervical nerve roots. Motor problems from median nerve compression usually show up as weakness in grip strength or atrophy of the thenar eminence (fleshy part of the palm near the base of the thumb). The median nerve and its branches innervate primarily the flexors of the wrist and fingers, as well as several muscles of the thumb. They include pain (often described as sharp, shooting, or electrical in nature), paresthesia ("pins and needles"sensations), and numbness. The sensory symptoms are located primarily in the palm (See Figure 1). Therefore, compression of the nerve may create both sensory and motor deficit. The median nerve carries both motor and sensory fibers. It is essential to thoroughly evaluate the problem before coming to a conclusion about the presence of the ever-popular CTS. We will follow the nerve's course from the spinal cord to its termination in the hand and describe common locations of compression pathology. This article will look at the entire length of the median nerve where there are numerous locations that median nerve entrapment may occur. Because this condition is studied so often, we have a very good understanding of how it occurs however, because it has become such a "popular" condition, clinical practitioners may be too eager to assume the presence of CTS simply because their patient/client experiences median nerve compression symptoms. CTS involves compression of the median nerve at the base of the hand in a region called the carpal tunnel. The most researched and well-defined upper extremity nerve-entrapment problem is carpal tunnel syndrome (CTS). ![]() ![]() ![]() His sermons are also often broadcasted on the Christian TV network TBN. ![]() Due to the peaked interest of new attendees, Pastor Todd added a service to his schedule to suffice the overflow. Over the recent years, the church's population has significantly increased. His language and style of communication make Todd stand out among other pastors and resonates with the audience. He preaches about topics relevant to the lives of millennials and Generation Z while staying true to God's Word. Pastor Todd's messages have been coined "the gospel of the New Generation" since most of his audience is the young adult generation. One of his most popular works is his original book "Relationship Goals How to Win at Dating, Marriage, and Sex." His best-selling book was a major trending topic amongst youth and young adults trying to navigate the dating world as Christians. His ability to incorporate modern methods of conveying God's Word and the latest social media tools to share the gospel is revolutionary for the Christian influencer realm. Todd is known for his passionate messages about popular topics for young adults that strays from the traditional church sermons. Their church aims to be diverse, progressive, and radical for Jesus. Todd's mission is to spread God's Word to every part of the world and transform lives through Jesus Christ. Since Michael Todd grew up in the church, it only fitted that he be the right fit for succession as head pastor. In 2015, Transformation Church was started under the guidance of Bishop Gary McIntosh, who turned over the honor to the married couple after 15 years of loyal servitude to the church and God. He is the head pastor of the Transformation Church in Tulsa, where he leads services alongside his wife, Pastor Natalie Todd. Times best-selling Christian author, and famous Christian influencer. ![]() ![]() ![]() Their most popular feature is the ability of users to connect with people from other countries. The best part is that you don’t even need an account to use their site, but if you decide to become a member you will get access to additional features. Just launch the app on your phone, tablet or computer and you are ready to go. You can connect to hundreds of strangers at any time of the day without leaving your house. They have millions of users from all over the world, and are always adding new features that make their video calling app better. Their main focus is on making online chatting easy and safe. ChatRandomĬhatrandom is the best random video chat platform for people who love to meet new people and chat with strangers online. Talking to strangers on a webcam always leads to some interesting conversations and you might even discover new passions, hobbies and maybe even new love stories! 2. You can meet people from your neighborhood or people who share your hobbies and interests, so you can make friends anywhere in the world! The best part is that you can talk with people from all over the world, so you will never feel lonely again. You can make free video calls right away to talk with strangers live on the internet. Other anonymous chat rooms are either very slow or have serious technical problems that make the video call experience unpleasant. There are a lot of random video chat apps out there but most of them are not as good as StrangerCam. StrangerCam is a website that lets you chat for free with strangers via webcam without any registration and it also has an app for your Android and iPhone devices, which makes it very easy to get started. What are the best random video chat apps? 1. Before you get started, read our guide on how to stay safe while using these apps. Here are seven popular live video chat apps that let you talk with strangers. ![]() Video chatting with random strangers can be a great way to kill time, meet new friends, and even find love. ![]() ![]() ![]() The rest of the band is solid, very good musicians. I never get tired hearing this voice sing. ![]() I'll just put it out there: The singing is spectacular! Very Robert Plant-ish, but yet different in his own way. , these guys are kicking off a classic rock revival. ![]() It is a double EP consisting of eight songs four from their debut EP, Black Smoke Rising, and four new tracks. "From the Fires is the second EP by American rock band Greta Van Fleet. Written-By – Daniel Robert Wagner (tracks: A1-A3, B1, B3-B4), Jacob Thomas Kiszka (tracks: A1-A3, B1, B3-B4), Joshua Michael Kiszka (tracks: A1-A3, B1, B3-B4), Richard John Thompson* (tracks: B2),Sam Cooke (tracks: A4), Samuel Francis Kiszka (tracks: A1-A3, B1, B3-B4) Producer, Recorded By, Mixed By – Al Sutton, Marlon Young Pressed By – Memphis Record Pressing – MRP1782Īrt Direction, Design – Ashley Pawlak, Kyledidthis Published By – BMG Rights Management (US) LLCĭistributed By – Universal Music Distribution ![]() Phonographic Copyright (p) – Republic Records SEALED NEW Copy ( no wear to cover at all ) Rory Gallagher – Defender (2018 Vinyl Reissue) € 24.Record Store Day 2019 "RSD First" release, including inlay with lyrics and credits. It surprises me how similar we all are.” Related products We’ve gone from driving down a highway to a show and seeing endless miles of shantytowns in Sao Paulo, to playing some of the richest places on earth.” Added Jake, “The more we’ve seen – different cultures, people, and traditions – we’ve also seen a similarity between ourselves and these different cultures. And then we were thrown out into this huge world, and it was a bit of a culture shock at first.” “We’ve seen how people live in different parts of the world,” added Sam, “and we’ve developed intrinsic respect for different cultures and people. As drummer Danny explains, “ We realized that while growing up, we had been shielded by a lot of things, we were unaware of a lot of things. The Battle At Garden’s Gate introduces a new chapter from the band – focused on fighting for what you believe in, self-empowerment, freedom, as well as the Internal and external battles of one’s self. Title : Greta Van Fleet – The Battles At Garden’s Gate. ![]() |
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